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Only when this intermediate of aggregation is significantly populated does the process of aggregation proceed to formation of large aggregates.
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This is affected by the concentration and type of accelerator and ultimately whether the aggregation proceeds according to RLCA or DLCA mechanisms [53, 55, 58].
Aggregation proceeds rapidly with no discernable lag phase and in a concentration-dependent manner (Figure S4; ThT assay method described in Text S1).
The apparent lack of a lag phase suggests either the presence of seeds or that aggregation proceeds along a downhill energy landscape, a phenomenon that has previously reported for a number of other aggregating peptides in which the critical nuclei can be as small as dimers [29].
In contrast, α1 became inhibitory after aggregation proceeded for approximately 6 hr.
Platelet aggregation proceeded faster in the presence of AGE and SHS.
As aggregation proceeds beyond a critical nucleus stage, a homochiral propagation process appears to be thermodynamically favoured.
Aggregation proceeds rapidly in vitro for each of these short sequences, occurring via stacked assemblies of high-order oligomers that populate extended, fibril-like conformers rather than compact or globular assemblies commencing even as early as dimeric species (Fig. 6).
Consistent with previously published data (Tam et al., 2009), mutant huntingtin aggregation proceeded rapidly and reached saturation 10 h after initiation of AcTEV™ protease cleavage of the GST tag (Supplementary Fig. 8A).
In order to determine at what concentration protein aggregation is induced by the varying salt solution conditions, the protein hydrodynamic radius was tracked using DLS measured particle size under identical buffer conditions (although not all conditions were replicated as at some of the higher ionic strengths aggregation proceeded too rapidly to accurately measure using DLS).
A previous study elucidated that small-sized platelet aggregates ultimately developed into medium-sized and large-sized aggregates as platelet aggregation proceeds.
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