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No aggregation or degradation of 125I-labeled chAb 14C5 was observed (approximately 150 kDa, Figure 2c, lane 2).
This can reduce or prevent aggregation or degradation, and alleviates the need for in vitro purification and reconstitution [18].
Further, receptor binding seemed to be induced or enhanced by oxidative modifications, aggregation or degradation.
The identification of different types of inclusions in neuronal and glial cells suggests cell type-dependent differences in DPR aggregation or degradation.
In addition, no aggregation or degradation or spectral changes of the ferric protein were observed during the time scale of spectroscopic measurements including RR spectroscopy (see below).
Ubiquitin is a small regulatory protein that plays an important role in the post-translational modification of numerous proteins, the addition of which often leads to protein aggregation or degradation [ 48].
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Moreover, ROS alter protein structure or function and Amino acid side-chains can be irreversibly modified into aldehyde or ketone groups (carbonylation) which can lead to protein aggregation, inactivation or degradation, these changes in protein carbonylation process are a biochemical perturbation resulting from oxidative stress [ 14].
Furthermore, expression of the relatively insoluble subunits of simple epithelial keratins carries the risk of protein aggregation induced cellular disease [30]in the event of mutation, imbalance of subunit expression, chemical induced aggregation or deficient degradation [31].
Aggregation or chemical degradation can be induced during sample preparation or purification.
It is possible that the expression of these miRNAs may relate to HTT aggregation or proteasomal degradation, as intranuclear inclusions are observed in pre-symptomatic HD [ 44] and the density of aggregate formation continues over the course of the disease.
This may reflect that the HSJ1a J domain mutant can still bind to, but not prevent the aggregation, or facilitate the degradation of mutant Parkin.
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