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This improved photodynamic activity might be due to the aggregation of compounds in the cell that form FONs which can promote PDT (photodynamic therapy) and are observed in cancer cell but not normal cell.
To suppress unwanted oligomerization and aggregation of compounds by shifting the equilibrium toward the monomers, we tried to find additives to inhibit SKF oligomerization and aggregation.
Therefore, NDSBs represent a class of cosolubilization reagents that directly resolve the oligomerization and aggregation of compounds for successful structural and biological analyses.
In addition, we found that cosolubilization of nondetergent sulfobetaines (NDSBs) reduced oligomerization and subsequent aggregation of compounds by shifting the equilibrium toward the monomers.
NDSB cosolubilization allowed us to perform successful structural and biochemical experiments with substantially fewer artifacts, which represents a strategy to directly resolve the problematic oligomerization and aggregation of compounds.
In addition, monitoring the oligomerization and aggregation of compounds under the same conditions as those of the assays is of importance, because the equilibrium of aggregation-prone compounds can change extensively in the presence of proteins or other solutes.
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UV Vis and FT-IR spectra suggested that hydrogen bonding between the different molecules was the main driving force for the aggregation of compound 2. In the AFM photos, there were some nano-aggregates formed in the 10−5 and 5 × 10−7 M solutions of compound 2.
Solid state FT-IR can also be useful in showing the ionic nature of hydrogen in the aggregation of compound 2 (Lee and Wang 2010; Das and Baruah 2011).
It was found that N, N-Dimethylformamide (DMF) was the best solvent for the Zn2+-triggered aggregation of compound 1 compared with other solvents.
These results supported the notion that hydrogen bonding played a key role in the aggregation of compound 2, which could simulate the hydrogen bonding interaction in the aggregation behavior of asphaltenes.
Aggregation curves of compounds of type I (A) and type II (B) are representative of 2 experiments with similar results.
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