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Seeding of intrinsically aggregated cells probably supersedes forced aggregation by centrifugation.
The % aggregation was the percentage of aggregated cells among the GFP-positive cells which transfected cells.
While the aggregated cells continued to show cell aggregation, higher cell hydrophobicity, EPS production, biofilm formation, PHB content and proline content in cells at 50 and 100 mM salinity, the values declined at 150 and 200 mM NaCl.
That is, shaking or repipetting the aggregated cells only partially dispersed the aggregates, leaving clusters of 10 cells or more.
Accurate cell counts of aggregated cells could not be obtained until the aggregates had dispersed, which required approximately 8 days.
A The aggregated cells showed suboptimal status.
The aggregated cells could withstand UV irradiation, heat, desiccation, different temperature regimes, H2O2 stress, and starvation with better population growth than non-aggregated cells.
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The pre-aggregated cells were then seeded into an in situ-formed hydrogel to generate MCSs.
However, further raising the temperature to 50 °C showed a slow but gradual and uniform decline in survival of both aggregated as well as non-aggregated cells.
Performance of aggregated and non-aggregated cells in this study even at 200 mM concentration substantiates the ability of Methylobacterium sp. to withstand higher salt concentrations.
Though the trend was similar in non-aggregated cells, at 200 mM NaCl concentration the IAA levels declined drastically (Fig. 4).
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