Exact(9)
Aggregate Quantification The httQ74-HA aggregation was detected by immunofluorescence (primary HA antibody).
The Resulting FOM is a single aggregate quantification of the multiple metrics on a normalized scale.
HCPs and culture medium components eluted later than mAb2 monomer and aggregates without influencing aggregate quantification.
However, DNA was detected only in the fractions containing host cell and culture medium components and not observed in the SEC fractions used for mAb aggregate quantification.
This study highlights that aggregate quantification directly in the cell culture supernatant using appropriate SEC columns with suitable mAb aggregate standards is feasible without falsification by previous affinity chromatography.
To ensure the absence of DNA and HCPs in the SEC fractions used for aggregate quantification, the corresponding fractions of the CHO mAb2 supernatant were collected (fraction volume: 98 μL) and analyzed.
Similar(51)
We developed a pulse-width flow cytometry method for aggregate detection and quantification.
Additional unique aspects include the use of aggregate FOM for quantification of performance, the use of Kiviat graphs as a visualization of CE behavior, and formulation of Taguchi methods within a CR application.
The quantification of aggregate intensities were carried out from SUM projected videos, and the intensity values were normalized to the surrounding cytoplasmic value.
Quantification of aggregate formation revealed that a huge amount of mAb consisted of aggregates.
Quantification of aggregate formation and LC3 dots were assessed as already previously described (16, 52).
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