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Cells that lack both HSP104 and HSP42 and display fragmented aggregate phenotype were mated with cells of the opposite mating type to reintroduce either Hsp104 and/or Hsp42.
If the aggregate phenotype in HG11 is due to a single locus, its function may be to disrupt energy partitioning by decreasing the deposition of lean tissue and increasing lipid accumulation in females, while having the opposite action in males.
Quantification of the aggregate phenotype identified an increase in the percentage of fish developing globular aggregates in skeletal muscle at 4 dpf, coincident with the disappearance of nemaline bodies (Fig. 3c).
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To directly confirm this expectation, we performed co-localization studies in cells transfected with pEGFP-MC-LT before the appearance of a completely aggregated phenotype.
Because the aggregates were formed by homogenous tumor cells, in terms of the multilineage phenotype, aggregate formation allowed for separation of these cells from other tumor cells in glioblastoma cultures.
Cells displaying a flocculation phenotype aggregate in the absence of EDTA and are dispersed in the presence of EDTA (data not shown).
In aggregate, any given phenotype has a certain degree of adaptation to each environmental factor so that the factor has its optimal intensity (e.g. optimal concentration) and extreme intensities, also called pessima (Fig. 7A).
The finding that tumors of a given gene expression subtype can express different sets of HOX-genes was particularly evident for the Genomically Unstable subtype and could indicate that the same aggregate gene-expression phenotype can be reached through different paths.
Pain perception is one of the most complicated measurable traits because it is an aggregate of several phenotypes associated with peripheral and central nervous system dynamics, stress responsiveness and inflammatory state.
For induction of a neuronal phenotype, aggregates were generated on bacterial-grade dishes and treated with 5 × 10−7 M all-trans retinoic acid (Sigma Aldrich) for 4 days.
We also provide a suite of more complex analysis techniques, such as aggregate functionality scores, phenotype-specific gene prioritization, and statistical methods for disease-gene finding in case/control studies without any need for data reformatting or computational expertise.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com