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The advantage is that the method can aggregate cells which include a little unevenness.
In this study, we focus on how the monolayer cells may affect different aspects of aggregate cells, which may have important implications for regenerative medicine.
We first developed a method to chemically aggregate cells, which involves a first treatment of cells with NaIO4 to produce surface aldehyde functionalities, and then incubation with acrylic acid-modified chitosan (chitosan-AA), a new inter-cellular linker.
The linker consists of a non-toxic low molecular-weight polyethyleneimine (PEI) backbone conjugated with multiple hydrazide groups that can aggregate cells within 30 min by reacting with the aldehyde handles on the chemically modified cell-surface glycoproteins.
Most of the cells making up the loose aggregate are undifferentiated and are referred to as aggregate cells (DDANAT:0000403).
They usually consist of a mixture of mono-dispersed and aggregate cells, spores, or viruses, carried by other materials, such as respiratory secretions.
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Figure 5 Aggregate cell throughput for best effort flows with different frequency reuse schemes.
The aggregate cell spectral efficiency achieved by the proposed schemes per frequency-time resource block is compared for both DAS and CMS architectures.
It has been demonstrated that, under the same total power constraint, the cellular DAS can double the ergodic aggregate cell spectral efficiency of the CMS.
The U.S. Centers for Disease Control and Prevention is also piloting a program to track aggregate cell phone location data in areas affected by Ebola to provide a better picture of disease reports in real time.
Aggregate cell movement was validated qualitatively for the multicellular scale model.
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