However, Ca2+-free incubation aggravated cold-induced cell injury in rat hepatocytes [ 46- 48] and liver endothelial cells [ 48] and Ca2+-free incubation was associated with increased ROS formation at 37°C [ 49].
In 542 A.D., the population was decimated by the plague, aggravated by cold damp conditions; the Black Death struck again between 744 and 747.
Subject N1 reported symptoms suggestive of asthma (cough, chest tightness, and wheezing aggravated by cold air, dust exposure, and upper respiratory infection), and her spirometry demonstrated mild airflow obstruction that improved slightly acutely following inhaled bronchodilator.
Oxaliplatin, an important chemotherapy drug active against colorectal cancer [ 1, 2], induces peripheral neuropathy triggered or aggravated in cold conditions even after a single administration [ 21, 22].
The husband is home with a bad cold and a bothersome obsession with air pollution, which he imagines is aggravating his cold.
"The urban construction patterns of New York City aggravate the cold air and make it even more difficult to maneuver".
Cosmetics can actually aggravate the cold sore further and slow down the healing process.
Although RPMI 1640 aggravated cell injury in the cold, there was no evidence of a toxicity of RPMI 1640: During warm incubation in RPMI 1640, which is the cell culture medium recommended by the German Collection of Microorganisms and Cell Cultures (DMSZ) for this cell line [ 29], Vero-B4 cells proliferated normally and showed normal morphology.
However, the enforced triple combination KHG(Ca--,P++) did not only aggravate cold-induced injury but also increased cell injury at 37°C during 14 h incubation (KHG: 10 ± 3%, KHG(Ca--,P++): 41 ± 20%).
Also, the choice of pausing medium for cell cultures and the solutions used in processing steps performed at lower temperatures should be carefully made, considering that some of the solutions severely aggravate cold-induced cell injury.
The southwest in particular is almost desert, with the dryness aggravated by a cold offshore current.
