It is known that allyl alcohol can be used as a select agent to isolate ADH-deficient mutants of some yeast species, such as S. cerevisiae [19], [24], K. lactis [25], [26] and C. guilliermondii [27].
Puromycin 2 μg ml−1 (Sigma) was used as a selection agent to isolate successfully transduced cells.
After the reaction, added immune separating agent to isolating the antigen-antibody complex, radioactivity of compound was determined and the standard combination rate was calculated.
An external agent invading the BW would induce a response from the epithelium that migrates beyond this agent trying to isolate it [ 2, 3, 23].
Conventional microbiological methods can fail to isolate agents because of their fastidious in vitro growth requirements or intermicrobial interactions; thus, for agent isolation, we used 2 culture-independent methods (agent-specific PCRs and 16S clone libraries) in addition to conventional bacterial cultures (17, 20 – 20 ).
Finally, government agents who had tried to isolate and intimidate him arrested him, took him to the airport and deported him.
Such agents may be difficult to isolate, identify, and treat effectively.
For example, using anti-EpCAM as capture agent, we were able to isolate single CTCs from artificial and clinical blood samples.
The department instructed any border control agents who encountered the man to "isolate, detain and contact the Public Health Service," Mr. Knocke said.
One remarkable document, the text of a report by a Soviet agent in Spain sent to none other than Kliment Voroshilov, Stalin's army chief, outlines a full strategy for going "decisively and consciously to battle" against the Republican government of Francisco Largo Caballero, the Socialist premier who, in the view of Moscow's agents, "is making desperate efforts to isolate the Communists".
