The phrase "age in vitro" is correct and usable in written English.
It is typically used in scientific contexts, particularly in biology and medicine, to refer to the aging process of cells or organisms in a controlled environment outside of a living organism.
Example: "The researchers conducted experiments to observe the effects of various factors on the age in vitro of stem cells."
Alternatives: "in vitro aging" or "cellular aging in vitro".
As the growth rate and proliferation potency of fibroblasts from subjects with HGPS decrease with cellular age in vitro and the donor's age, this could partially explain the differences between our results.
In the present investigation, AM, OT and FF were found to inhibit the formation of AGE in vitro.
While this may be due to donor-related issues such as age, in vitro technical aspects including harvesting and preservation procedures that may also drive these differences.
In fact, the mechanisms of iPSC aging and its opposite (rejuvenation) during somatic cell reprogramming are mostly unknown and finding features that efficiently measure age in vitro is one purpose of this project.
We found that at this age, in vitro preparations also produce robust rhythmic output from lumbar motor pools that innervate abdominal muscles active during expiration, thoracic motor pools that innervate intercostal muscles during expiration, and VII motor pools, all of which is consistent with previous work (Huangfu et al., 1993; Iscoe, 1998; Onimaru et al., 2006; Iizuka, 2010).
These results indicate that naïve HGPS stem cells are capable of differentiating into SMCs in vitro and inducing the accumulation of progerin in HGPS-SMC nuclei with increasing cellular age in vitro; as reported in recent HGPS-iPSCs studies (Liu et al., 2011; Zhang et al., 2011), and in our previous in situ studies (McClintock et al., 2006; Olive et al., 2010).
Samples were subsequently aged in vitro at a constant temperature in artificial saliva supplemented with food colouring for two cycles of 14 days each.
The data also provide biological insights into which cell characteristics (proliferation and adhesion, for example) are functionally clustered and either change or remain constant with aging in vitro.
When similarly studies were performed with normal fibroblast cultures, we noticed that the number of cells positively labelled with anti-progerin Ab increased slightly with the cellular-age in vitro.
Though lack of a clear correlation between organismal aging with cellular growth viability, the study of mammalian cell aging in vitro has enormous potential for telling us how human aging works [1].
We observed that immediately fertilized eggs from the second spawn showed also a high loss in development potential like eggs from the first spawn aged in vitro for 3 hours (Fig. 4A B).
