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The antibacterial effects were determined using in vitro anti-bacterial analyses by a fluorescence staining method employing Syto9 and bacterial viability agar tests.
Although four different types of chromogenic agar were used across the studies, the clinical findings compared with PCR tests were consistent for all chromogenic agar tests.
These results indicate that B. breve B632 exerted antimicrobial activity against coliforms in fecal cultures as well, consistently with previous observation with spot agar tests and cocultures [ 24].
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CTAB methylene blue agar test is a simple method for the detection of anionic surfactants.
The strain P4 was screened by various methods including drop collapse assay, CTAB blue agar test, emulsification index assay and BATH assay.
The following analyses: CTAB/methylene-blue agar test (Siegmund and Wagner 1991; Kayganich and Murphy 1991); Biuret test (Feigner et al. 1995); and phosphate test (Okpokwasili and Ibiene 2006) were carried out to determine the class of the biosurfactant produced.
The direct inhibition of infection by plant pathogen, F. oxysporum on V. radiata (moong dal) and C. neri (black chickpea) in the presence of silver nanoparticles was conducted using dual agar test (DAT) test [35].
The BS producing ability of the isolate P4 was studied using standard parameters, described earlier such as foaming test (Ferhat et al. 2011), drop collapsing test (Tugrul and Cansunar 2005), emulsification index (Deepak and Jayapradha 2015), BATH assay (Rosenberg 1984), cetyl trimethyl ammonium bromide (CTAB) agar test (Satpute et al. 2010).
Means of V. longisporum-DNA amounts determined by qPCR were significantly correlated with the colonisation values of the malt agar test in E3.
Presumptive MRSA colonies were confirmed to be S aureus by DNAse agar testing and positive agglutination reaction using the Pastorex Staph Plus Kit (Bio-Rad).
Images of ∼18-h yeast colonies on all replicate YPD agar test plates were captured with a BioSpot prototype machine at 1024 by 768 pixels and archived.
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