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The behavior is different in the uncoated area of the substrate and in the rest of the agar slab where the growth of colonies is high as in the reference sample (Figure 6a). Figure 6 Bacteria growth.
The first one (Figure 6a) shows a reference substrate (a bare glass slide) and it is clearly seen that a high number of Lactobacillus plantarum colonies grown up randomly throughout the whole agar slab.
(A ) Confocal images of A375 cells confined between uncoated glass and an agar slab.
(B – H ) Images and analyses of cells plated on glass and confined under an agar slab.
(B – I ) Images and analyses of A375 cells plated on glass and confined under an agar slab.
(A – I ) Images and analyses of A375 cells plated on glass and confined under an agar slab.
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Figure 6 shows the results of two samples placed on agar slabs after 24 h.
The two agar slabs sandwiching the roots were placed vertically in a dark box.
Roots were allowed to grow at 25 °C in the dark for 4 days, attached to both agar slabs.
This involved germinating japonica rice (Oryza sativa) caryopses sandwiched between two agar slabs with or without 1-aminocyclopropane-1-carboxylic acid (ACC) at 1 µM.
Germinating rice caryopses were sandwiched between two 2%% (w/v) agar slabs 12 mm thick containing 1/10 strength Hoagland medium (Sigma-Aldrich Japan K.K., Tokyo, Japan).
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