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Vertical Agar Plate test.
Fig. 3 Agar plate test for fungi.
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The tablets of each group and a control group were subjected to the agar plate diffusion test to measure the zone of bacterial inhibition.
When testing almost 300 S. aureus isolates collected in Swedish surveys on CM and SCM only 31%% of the isolates were positive in the Congo red agar (CRA) plate test which indicates slime production (K. Persson Waller, unpublished data).
The strains were stored at −80°C in trypticase soy broth supplemented with 15% glycerol and were passaged twice on a blood agar plate before testing.
The one MRSA strain that did not grow on the Oxoid Brilliance™ MRSA agar plate was tested for its cefoxitin susceptibility using disk diffusion.
At least 1 E. coli colony was randomly selected from each MMH agar plate and tested for disk susceptibility to 24 antimicrobial agents by using Clinical Laboratory Standards Institute (CLSI recommended methods, interpretive criteria, and reference strains (15 ).
Isolates tentatively identified as E. faecalis or E. faecium based on appearance on the Flexicult agar plate, but testing negative in the E. faecalis- / E. faecium-specific PCR (n=9), were characterized by 16S rRNA sequence analysis (Macrogen®, Seoul, South Korea) [ 14] using primers modified after [ 15].
Alkane susceptibility test on agar plate: Alkane susceptibility test on plates was performed according to the methods of Mauersberger et al. [ 28, 29].
From a second subculture on agar plates, a test suspension was adjusted to 1.5 5.0 × 10 colony forming units (cfu) per ml.
Transformants were selected on maltose agar plates and tested for growth on various carbon sources in serial dilutions.
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