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Phospholipase production was estimated by using an agar plate method.
Izumida, H., Miki, W., Sano, H. & Endo, M. Agar plate method, a new assay for chitinase inhibitors using a chitin-degrading bacterium.
In this study, the screening of lipase positive bacteria from rice flour was carried out by Rhodamin B agar plate method.
(a) Symbol open circle represents data obtained from agar plate method.
This was compared to CFU obtained using agar plate method to demonstrate the utility of the test.
Solid line (CFU equivalent from MBRT) and the symbol open triangle (data from agar plate method) are for an initial cell concentration of 104 cells/ml.
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Further, the methodology should indicate the presence of a contaminant in short time periods since in the agar plate methods used frequently it takes about 24 h.
In order to obtain directly colonies for further isolation, agar plate methods were preferred over MPN procedures with liquid media for determining concentrations of fecal bacteria.
TVC was conducted on nutrient agar media by means of serial dilution agar plating method (Marzan et al. 2017).
The Rif- and INH-resistance of the same culture were also evaluated by the agar plating method for comparison.
As shown in Table 3, the Alamar Blue dye method yielded antibiotic-resistance values that broadly correlated with the resistance values estimated by the agar plating method.
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