However, these studies were performed using traditional manual enrichment broth/solid agar culture methods, only sub-culturing when broths were cloudy or after a fixed time period.
We found the agar colony culture method to be most suitable and methodologically most advantageous.
Samples were processed by the various reference clinical microbiology laboratories of the participating hospitals using standard procedures, including Sabouraud agar culture and BACTEC method (Becton Dickinson Diagnostic Instrument Systems, Paramus, NJ, USA), for the isolation of fungal species.
In accordance with the method described by Araki et al. [ 28], we plated the bacteria directly on the surface of selective agar (spread-culture method) and exposed them to a continuous stream of native whole (WS) and filtered cigarette smoke (GVP) of four cigarettes (K3R4F, C1, CM5, K1R5F) with different tar content (ranging from 1.67 to 15 mg per cigarette).
The combination of Lim broth and subculture on Granada agar was the only culture method that detected all GBS positive women.
The visual agar plate assay and tip culture method were used to determine the range and extent of antifungal phenotypes.
By visual agar plate assay and tip culture method, the strain JPP1 exhibited remarkable inhibitory effect on mycelia growth (antifungal ratio >95%) and subsequent aflatoxin production (antiaflatoxigenic ratio >98%).
Soft agar culture was performed using standard methods.
Using the spread culture method [ 28], the bacteria were poured directly onto the selective agar plates (composition: 1.5% agar, 2% glucose, Vogel/Bonner medium) already complemented with a histidine/biotin solution (0.5 mM).
In this culture method, a segment of the catheter tip is rolled across a blood agar plate to detect the presence of bacteria on the external catheter surface.
By conventional culture method, there was no growth of Shigella on the LB, MacConkey and SS agar plates.
