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This was analysed by using pair-wise Blast bit scores normalised against alignment length; see Methods for further details.
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Coverage of greater than 6× was obtained for each sequence, and nucleotide identities were manually checked against alignments.
Sequences were also checked against alignments of all target gene sequences deposited in GenBank for the species of interest to check in silico sensitivity.
We then used MuMMer 2.3[ 31]to align the contig sets for each strain, using an all-against-all alignment to identify contigs that were similarly constructed between the assemblers.
This process was repeated three times in order to only remove sections in inverse proportion to how well they aligned, balanced against the alignment quality.
The random databases were analyzed in the same all-against-all fashion, and the resulting scores were compared against real alignment scores.
It was effective: Batters hit.186 against the alignment with a.339 slugging percentage.
Many groups mediate between high schools and colleges, but they have competing agendas that tend to work against curricular alignment.
The carbon nanotube film heaters developed in house were built for the first time on a counter-intuitive concept to pass electric current against the alignment axis.
In each case, an ELK group was compared against an alignment of all other ELK groups.
BAliBase [ 22, 29] was the first large scale benchmark dataset against which alignment programs were routinely assessed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com