During his month-long trip, Slim also visited the New York Public Library and the Statue of Liberty, and he went again and again to the New York Stock Exchange, burying himself in its library.
To further explore the apparent change in promoter specificity of this polymerase, we carried out an additional screen in which R3 17 was again cloned adjacent to the library of promoter mutations, and replica-plated colonies that grew poorly or not at all upon IPTG induction (indicating polymerase overproduction) were picked and sequenced.
The numerous negative supernatants could correspond to plasmids carrying no or partial inserts and again beginning to overwhelm the library, to scFvs with weak affinities or to scFvs having a low expression level in this format.
Time and again I come back to the library as where I got my real education, and The Way of Terry Pratchett is this: you go through the very, very top of a very big library and you read every last book, which effectively is what quite a lot of my adolescence was made of.
The results were quantified with a Qubit 2.0 Fluorometer (Invitrogen) to confirm the presence of DNA, which were amplified using a Ion Plus Fragment Library Kit (Life Technologies) and purified again to produce the mtDNA libraries for each sample.
But they'll have to, to finance the library again next year (and the art program, too, has no money for the 2013-2014 school year).
Then to the library again, but I left in the afternoon to come home and write in white Helmut Lang jeans and a blue Rag & Bone button-down.
Now I would never again be able to return to the library nor would I be able to finish the illustrated series of classics.
Partly because public libraries are often seen as a soft target; partly because they say local authorities consistently undervalue the breadth of what they do; and partly because the cutting will be done during a recession, which is exactly when everyone starts going to the library again.
A month later, the magazine's opening page once again pressured the library to lift its tobacco ban: Smoking as a habit with both sexes is now a generation old.
