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Hours later he was once again removed to Mexico.
The aqueous layer was again removed to a fresh tube containing 400 μl of isopropanol, and then the DNA was precipitated by centrifugation at 14,000 g for 10 minutes.
After DNA degradation, the same volume of phenol chloroform mixture was added to the samples and centrifuged at 15,000 × g for 10 min. Supernatants were again removed to new tubes and the same volume of 2-propanol was added and centrifuged at 15,000 × g for 20 min to pellet DNA.
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On April 18, it was again removed, only to be re-chalked.
Five days after administration of DepoProvera, mice were anesthetized (as described previously) and vaginal mucous was again removed prior to infecting vaginal lumen with 2,400 plaque forming units (PFU) HSV-2.
The upper layer was again removed and added to the first organic layer.
LoS contributions have been again removed, in order to achieve a more reliable representation of the actual scattering patterns.
The middle layer was again removed, glycerol was added to 5% final, and the extract was aliquoted and flash-frozen in liquid nitrogen.
Montana State University-Northern plans to ask the state Supreme Court for an order allowing the university to again remove Kevin Emerick as woman's basketball coach.
His gloved fist fumbled to again remove his gum shield, a gesture that in another fighter would have signalled that he had had enough.
Again, remove and rest.
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