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Then, F0 zebrafish were outcrossed with WT zebrafish and the F1 progeny were genotyped and outcrossed again for two generations, followed by an incross to obtain homozygous mutants (Fig. 1D).
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"Then nobody touched language for two generations.
The Greatest Generation dominated politics for two generations.
The name alone meant royalty in Nassau County for two generations.
Farrakhan has been wielding major political influence for two generations.
Latin has been getting abolished now for two generations".
"You'll borrow for two generations to sue him..
These lines were inbred for two generations.
The resultant progeny are backcrossed again with LG o2 for five generations and then self-crossed for at least three generations to obtain a modified LG o2 mutant (LG Mo2).
It's been there for three generations.
Our family's been fishing for four generations," she said.
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