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After integration of the selection marker, a second plasmid carrying a mutation cassette, again flanked by I-SceI sites, is introduced.
In libraries stem-(1), stem- 2), stem- 2) and stem- 50), 1,2,3,5 or 10 additional restem- 5and instem- 10nto the randomized EF loop, without stem- 10ing mutations, while the EF loops in the corresponding libraries stem(1,2,3,5m(2), stem(3), stem(5) and stem(10) are again flanked by tworstabilizing mutations.
Similarly, at another developmental stage, when a different set of transcription factors are expressed, unit 1 might be transcribed in factory C. It is again flanked by units 2 and 3, but these can now be transcribed efficiently only in factory B (which is rich in the necessary factors).
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The fishtank globes, bubbling again, flanked the bar.
He retreated to the dugout before emerging again, this time flanked by two police officers as two other officers walked in front of the spectator on top of the dugout.
They did not stay long, however, and set sail again, this time flanked by a naval task force.
Dennis's uncle, Derek Oland, was once again in attendance, flanked by his sons, Moosehead president and CEO Andrew Oland, its chief financial officer Patrick Oland, and many cousins.
Dennis' uncle, Derek Oland, was once again in attendance, flanked by his sons, Moosehead president and CEO Andrew Oland, its chief financial officer, Patrick Oland, and many cousins.
The highway meanders again through lake country flanked by residential subdivisions of the township.
1315 Tony Blair, flanked by George W Bush and Jacques Chirac, again condemns the attacks.
In this case we could also measure construct-carrier concatenation and observe that it was at such a level that almost all construct molecules would be flanked by carrier molecules (again about 90%, considering two possible orientations for the carrier molecules).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com