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As alignment errors can create false positives in the detection of positive selection footprints, each cluster suggested to be under positive selection was again checked both automatically – using muscle [ 72] and trimAL [ 73] for creating and cleaning alignments (muscle-trimAL method; see Additional file 1) – and manually for alignment errors.
The used catalysts were again checked by XRD and UV Vis reflectance spectroscopy to explore the stabilities of samples.
All acquisitions were checked again using the Statistical Parametric Mapping (SPM8) software (http://www.fil.ion.ucl.ac.uk/spm/) by registering all DTI images to their first b0 images.
After SILVA-ngs analysis, the largest OTU of Cyanobacteria (containing 287 reads) detected at 3000 m were manually checked again using the web-BLASTn against the NCBI non-redundant nucleotide database.
Again, check the terms.
Again, check the labels.
Again, check your pulse.
Again, check for leaks.
Again, check eBay.
Again, check their references!
Again, check your heart rate.
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