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All sequence data were again aligned to these new consensus sequences, updated reference sequences were again created, etc.
Contigs that contained BAC-ES were filtered and again aligned to the G. aculeatus genome as described above.
Raw reads were again aligned to the set of 3310 transcripts with a mapping quality filter of q = 55 to ensure mappings were unique.
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We then cut them in half in silico and again aligned them to the human genome and determined the fraction of derived alleles.
The assembled contigs from all of the clusters were used in place of the reference in the second iteration, and the Rmaps were again aligned and assembled to produce a new set of Rmap contigs.
Once again, the LyTASK sequence was aligned to this, now a mix of templates, via the ClustalW algorithm.
The trimmed paired-end reads were again aligned, and as detailed in Table 1, 71.75% of the reads mapped to the NL4-3 reference genome, with 59.15% mapping in pairs.
Santorum has once again aligned himself with his ever-present Bible and selected the name "Petrus" which refers to St. Peter.
In the 21st century, some Russian feminists, such as the punk rock band Pussy Riot, have again aligned themselves with revolutionary anti-government movements like the 2012 demonstrations against President Vladimir Putin, which led to feminism being called a "mortal sin" by a lawyer representing the Russian Orthodox Church.
I found, once again, aligned claims across the four newspapers and between politically opposed policy-makers.
Data was again aligned on a common spherical coordinate system.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com