Sentence examples for afterwards stimulated from inspiring English sources
For this purpose cells were positively selected by antibody-coupled magnetic beads and afterwards stimulated with the virus.
To assess the ghrelin and leptin induced response, cells were perfused with Krebs buffer for 12 minutes and afterwards stimulated with ghrelin (10−10 M; Peptide Institute, INC) or leptin (10−12 M; Phoenix Pharmaceuticals) for 20 minutes.
In pre-treatment experiments, cells were first incubated for 10 minutes with 200 μmol PC, washed, and afterwards stimulated with TNF-α (10 ng/ml) only.
The presence of these genes was compared to the expression pattern of the Oct4 transcription factor, which is present in the early cleavages, repressed after this initial stage, and then its expression is afterwards stimulated again in the blastocyst [ 22].
The cells were lifted, centrifuged, and cultured under serum-free conditions for 24 hours, and subsequently pre-inhibited for one hour with 100 mM 3-isobutyl-1-methylxanthine, IBMX (Sigma-Aldrich, UK), and afterwards stimulated with 1 pM-100 nM salmon calcitonin for 15 minutes.
Afterwards, cells were stimulated with 5 ng/ml TGF-β1 for 24 h and lised for western blot analysis.
Afterwards, cells were stimulated by addition of 25 μL Earle's medium containing opsonized zymosan at a final concentration of 0.026 mg per well.
Afterwards, cells were stimulated with 10 μM S1P for 2 min and then lysed for protein extraction and further assay procedure according to manufacturer's instructions.
There were issues that some participants couldn't or wouldn't raise in front of the family, but also others that arose afterwards having been stimulated by the meeting.
Afterwards, they were stimulated with viable Salmonella serovar Typhymurium DT104 at a multiplicity of infection (MOI) of 10 in triplicate. 2 h post stimulation adherent as well as non-adherent cells were washed twice with warm PBS and lysed for total RNA isolation.
Afterwards, each volunteer was stimulated with a 1.6 milliamp current, or assigned to a control group that received the same procedure with the electricity switched off.
