Exact(4)
Two days before launch, the ECs were cultured in the EUs, which were afterwards mounted on the SpaceX CRS-8 rocket.
Slides were counterstained with hematoxylin (Sigma-Aldrich), dehydrated and afterwards mounted with Histomount (National Diagnostics).
For cryoprotection, blocks were infiltrated overnight with 2.3 M sucrose at 4°C and afterwards mounted on aluminum pins and frozen in liquid nitrogen.
After fixation of the human and animal meniscus samples in 90% methanol in 4°C for 48 h, specimens were infiltrated overnight in PBS with 5% sucrose at pH 7.4 and afterwards mounted on chucks in Jung tissue embedding medium (Leica, Germany), frozen in a HM 500 OMV cryostat (Mikrom, Germany) and cryosectioned at 12 μm.
Similar(55)
Shortly afterwards, a mounted infantry charge by the Australian Mounted Division's 4th and 12th Light Horse Regiments (4th Light Horse Brigade) with bayonets in their hands, their only weapon for mounted attack, as their rifles were slung across their backs.
Wings, pupal cases and pupae of these specimens were mounted afterwards.
Afterwards, they were mounted in glycerol on a slide, covered with a cover slip and sealed with nail polish.
Afterwards, worms were mounted onto 3% agarose pads containing 20 mM sodium azide and observed with a Zeiss Axiovert 25 microscope.
Afterwards, slides were mounted and, using the same optical microscope mentioned above, the number of epithelial cells with brown stained nuclei in the corneal basal layer was determined in five serial sections of samples from each animal.
Afterwards, cover slips were mounted on microscope slides using anti-fade mounting medium (Sigma Chemicals).
Afterwards the sections were mounted, dehydrated, cleared, and coverslipped.
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