To construct libraries of expressed DNA sequences, RNA was extracted from 2-yr-old oysters, from G3 inbred lines 51 and 35, after we subjected small groups to different stressful conditions.
The author teaches his students that we all become furniture after we die, subject to the same environmental changes an old bed or chair is.
At 45 days after treatment, we subjected pancreata to histological analysis and evaluated total β-cell mass followed by immunostaining with insulin Ab on serial pancreatic sections.
In order to confirm some of the novel introns, we designed primers flanking the intron region and, after amplification, we subjected the PCR products to Sanger sequencing.
At 48 hr after infection, we subjected the cells to subsequent treatments with Cr VI) or harvested them for Western blot analyses.
We first assessed the boldness of all fish by recording their behaviour in isolation, after which we subjected them to the same environment again but this time allowing observation of a conspecific through a transparent partition.
To determine the possible functional role of proximal UPR signaling components in locomotor recovery after SCI, we subjected ATF4 knockout mice (atf4−/−) and littermate controls to spinal cord hemisection.
In order to study how damaged brain tissue interacts and may be replaced by newly generated cells after injury, we subjected adult flies to penetrating traumatic brain injury, by lesioning the optic lobe (OL) unilaterally with a thin metal filament.
After we asked subjects detailed questions about their three most recent partners, we asked about the total number of partners subjects had beyond those three from age 13 to 19.
After we asked subjects these detailed questions about their three most recent partners, we asked about the total number of partners subjects had beyond the three most recent partnerships from age 13 to 19.
