Sentence examples for after transformed from inspiring English sources
Screening positive cloning after transformed into DH5α competent cells and identified by PCR amplification and DNA sequencing.
After transformed with pET22b-VHH-His, E. coli BL21 cells were grown in 1L LB medium containing 100 μg/ml ampicillin at 37 °C overnight.
After transformed into E. coli strain BL21 (DE3), the cells were grown in either LB medium for preparing unlabeled protein, or M9-minimum medium with 15NH4Cl and/or 13C-glucose (Isotec, Miamisburg, OH) as the sole nitrogen and/or carbon sources for preparing isotope-enriched protein.
After transformed into E. coli DH5α, recombinant plasmids were selected on Luria-Bertani agar containing 25 μg/ml zeocin.
After transformed in competent Escherichia coli K12 strains were fermented in batch and fed-bach mode in different mediums in order to select the best conditions of expression.
After applying the power transformation, the remaining variation was highest in Germany, with a variance after transform of 17%.
More than 100 positive transformants were obtained after transforming the silencing construct into C. reinhardtii CC425.
After transforming, the positive C. tropicalis transformants co-expressing xylanase and xylosidase produced larger hydrolysis zones than those expressing xylanase alone, when incubated on xylan-congo red plates.
The geometric mean of CMFL was calculated after transforming the count data using log (x + 1) transformation [ 12] and p-value < 0.05 was considered significant during the analysis.
After transforming the pCB1532::MPG1 into ΔpdeH mutant, the SUR resistant transformants were screened by qRT-PCR with primers MPG1_QF/MPG1_QR.
After: "Transforming consumers into fans".
