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*P < 0.05, **P < 0.01, ***P < 0.001 Fig. 6 Brc susceptibility to vancomycin after transference to planktonic mode of growth.
After transference, membrane blocking was achieved by incubation in AP-T buffer (1 M Tris/HCl pH 7.4, 1 M NaCl, 25 mM MgCl2, 0.03% (v/v) Tween 20) containing 5% (w/v) milk powder.
Open image in new window Fig. 5 a Raman spectrum of CVD graphene (excitation wavelength 532 nm) after transference to SiO2 (300 ± 5 nm)/Si (black curve), after vacuum annealing at 450 °C for 1 h (red curve) and ITO nanoparticles-decorated graphene (blue curve), zoomed spectra of Lorentzian-fitted graphs of the b G peak, c 2D peak, and d peak.
Equivalent loadings were verified by Ponceau Red staining after transference.
After transference the membrane was blocked with milk 5% in TBS-Tween for 1 hour.
For secretome samples, equivalent loadings were verified by Ponceau Red (Sigma) staining after transference (data not shown).
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After protein transference using a semidry electroblotter, PVDF membranes (Immobilon P, Millipore) were incubated with an antibody against S100A4 (DAKO), and detection was accomplished by secondary horseradish peroxidase-conjugated antibody and enhanced chemiluminescence, as recommended by the manufacturer (Amersham).
Embryos were obtained by somatic cell nuclear transfer, and, after 6 transferences to recipients, 3 pregnancies and 1 viable bitransgenic calf were obtained.
After describing motion as the transference of a body from the surrounding neighborhood of bodies, Descartes states that by "one body, or one part of matter, I here understand everything which is simultaneously transported" (Pr II 25).
Reduction of touch transference was measured after 0 m of drying and reduction of persistence was measured after 90 m of drying was added in combination while each time point was sampled using RODAC plates.
Newborn data will be collected using the babies' medical charts, which will be evaluated only after their discharge from hospital, transference or death, upon the mother's informed consent.
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