Sentence examples for after thrice from inspiring English sources

But the stories are great: they include one by Allison Yates, who, after thrice being laid off, has reluctantly accepted her true calling: being a housewife.

After thrice washing with 350 µL washing buffer, invert the plate and blot it against clean paper towels.

As shown in Fig. 5, the interfacial tension of surfactant polymer was increased to 10−1 mN/m after thrice absorption.

After thrice washing with 350 µL washing buffer, add 90 µL of TMB substrate to each well and incubate for 30 min at 37°C in the dark.

However, Innocent XI backed down after thrice failing to achieve the support of the majority of his cardinals for a bull banning nepotism, which had been tediously composed between 1677 and 1686.

After 72 h, viable cells were harvested, and, after thrice washing with HBSS, the cells (1 × 105 cells/200 μl/well) were stimulated in 96-well flat-bottomed plates (Falcon Labware) coated with 5 μg/ml anti-mouse CD3 mAb.

The first man to lead Europe did so only after Antony "thrice presented him a kingly crown, which he did thrice refuse".

Well, after Remington Steele, James Bond thrice, The Matador and Mamma Mia!, it turns out that Pierce Brosnan – ridiculously well-preserved or otherwise – is, with The November Man, finally old enough to admit that he's old.

After washing thrice for 5 mins each with 0.01 M PBS, sections were incubated in blocking solution containing 10% normal goat serum (NGS) (for vGluT, NeuN, GAD67), or 10% normal donkey serum (NDS) (for vGAT, PV, somatostatin) (or both for double labeling) in 0.25% Triton-X in 0.01 M PBS (PBST) for 1 hr at room temperature.

After washing thrice with PBS, the sections were then air-dried, dehydrated, and mounted onto cover slips for light microscope observation.

Dubbed the Sarkozy effect after the thrice married president of France, the U.K.'s Harley Medical Group reported a more than 50% increase in the number of men aged 35 to 55 having Botox treatments in 2008 compared with 2007.