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Kinetic traces proceeded for 30 min, and initial velocities were recorded after the trace had attained linearity.
For each single cell time-trace, a moving average smoothing filter is applied to remove any technical noise and the maximal YFP value is determined after the trace has reached a plateau.
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After this, the trace returned to a wavy pattern at 1545 JST.
After monitoring the trace for 5-min, we took one more reading of the seal resistance, checked the baseline trace once more, and began data collection.
After inspecting the trace files generated by the Bayesian Markov chain Monte Carlo (MCMC) runs, we determined that the initial 25% (2,500) of sampled generations would be omitted.
After removal of the trace amounts of DNA contamination with DNase I, total RNA was reverse transcripted into cDNA with kit from Life Technologies (Grand Island, NY, USA).
After that, trace the photo on the center, and cut about 1/8" in from the outline of the photo to avoid having the edges show.
After that, the traces of solvent are removed by evaporation.
After collecting the traces for the receiver and interferer channel signals, we consider the area below the intersection of these traces in relation to the power spectral density of the interferer signal.
Therefore, HEt data, MitoSOX and Bodipy data were analysed by determining the slope of the trace after normalization to the first data point.
The mean velocity of PVVel and HAPI were calculated automatically by the machine after the waveform trace for three cardiac cycles were obtained.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com