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After the sequencing reaction, the reaction product was loaded onto an ABI 3700 Capillary Sequencer.
On May 3 , 2012 nearly two years after the sequencing study began, the Mights met with the Duke team in an examination room of a children's hospital in Durham.
After the sequencing step, extension products were size-fractionated by capillary electrophoresis and sequences were compared to the M2 receptor gene reference sequence from the Ensembl genome database using SeqScape (Applied Biosystems, CA) and BioEdit (Ibis Therapeutics, CA) softwares.
The high coverage achieved for all tissues after the sequencing of 40,000 to 63,000 phage amplicons in this round of synchronous selection [29] strongly suggests that most of the diversity in these tissues has been covered (Figs. 4A D).
Sets of high-confidence tissue-specific peptides along with improved statistical analysis of longer motifs can be undertaken after the sequencing of phage DNA recovered from a large number of tissues, as well as from specific tissue samples recovered by micro-dissection from paraffin-embedded tissues.
After the sequencing, raw sequence data were generated by Illumina RTA 1.12.4.2 and CASAVA-1.8.2.
Similar(12)
For example, what number logically comes after the sequence of 1, 2, 4, 7, 11?
After the sequence of each fragment is determined, powerful computers assemble the pieces in the correct order by looking at overlapping sequences.
The Madison Square Garden crowd knew the significance of the moment, too, and they cheered Chandler immediately after the sequence.
Sequence awareness after the sequence learning task was tested by means of a questionnaire and the process-dissociation-procedure.
After the sequences were submitted, a relatively rich database is now available for comparison.
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