Sentence examples for after the second passage from inspiring English sources

For this comparison MSC were harvested after the second passage (P2).

After the second passage, melanocytes were maintained in RPMI-1640 with 10% FBS for at least 48 hours prior to harvest.

MSC from different-aged donors were harvested after the second passage to determine age-induced changes in their gene expression profiles.

However, after the second passage, differentiated structures as "cord-like" structures, colonies expressing red pigmentation or other kind of defined-cells appear under the microscope intermingled with the scattered GFRa2+ cells.

For stimulation and RNA isolation, cells were grown in 12-well tissue culture plates (BD Biosciences) and were used after the second passage at a confluence of 70 80%.

For stimulation and RNA isolation, cells were grown in 12-well tissue culture plates (4 cm2/well; BD Biosciences) and were used after the second passage at a confluence of 70 80%.

The monolayers after the first and the second passage were read for BRSV specific cytopathic effects.

Free floating cells (absorbing Trypan blue) from the medium showed IDH1 mutation after the first and the second passage.

The amount of virus produced after replication of Sabin poliovirus type 1 in K562 cells did seem to increase after the first passage, suggesting that replication of the passaged virus is more efficient than replication of the parental virus.

After freezing, gene expression of the second passage is comparable to gene expression prior to freezing.

By last summer, just three years after the first passage, 50 ships crossed above Russia, including eight tankers chartered by Novatek to test the route.