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Finally, using as criterion for defining the 5' end of a transcript the location of a SL addition site, most of the polycistronic transcripts obtained after the initial assembling could be split up, giving a total number of 10 285 transcripts (Table 1).
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The initial assembling generated 253 contigs, from which 140 contigs remained after excluding non-OR sequences and OR sequences with <5× coverages as well as unifying sequences with <1% sequence divergences (table 2).
The initial assembling gave rise to 195 and 194 contigs from genomic DNA and cDNA, respectively.
From the initial assembled scaffold covering 255 Mb sequence data, 28,602 SSRs (≥5 repeats) were identified.
After the initial separate assembly, the contigs assembled and the remaining unassembled sequences were subjected to a secondary assembly using MIRA.
After initial assembling, it was possible to define a total of 6937 transcripts; a number lower than the 8272 protein-coding genes previously predicted to exist in the L. major genome [ 13].
After the initial adapter trimming and quality filtering, we assembled the remaining 241,170 cleaned reads using Celera assembler [ 18] and obtained 8,422 contigs and 60,910 singletons.
A de-Bruijn graph building approach was used for primary assembling in the initial stage of assembling, which provided long assembled sequences.
After the initial craze, the cube crashed.
After the initial panic, little changed.
After the initial shock, it fits eloquently.
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CEO of Professional Science Editing for Scientists @ prosciediting.com