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Detailed information about the expected sequence of events after delivery was appreciated.
After PCR amplification using sunflower genomic DNA as template, products with the expected sequence were obtained for 10/19 loci.
Plasmids isolated from clones which contained the expected sequence after sequencing confirmation of the insert were used in further experiments.
Sequence analyses confirmed the expected DNA sequence.
Four TUs (TUs 3, 8, 12 and 15) yielded non-specific RTPCR products which did not map to the expected locus after sequencing.
Products of the expected size were sequenced.
Clones with the expected structure were sequenced.
After trimming of adapter sequences, most sequences were of the expected size of 20 or 21 nt.
DNA sequencing of PCR-amplified product after the pull-down with the STAT3 antibody showed the expected CNTF gene sequence.
Plasmids showing inserts of the expected size after restriction were selected and sequenced by CoGenics (Grenoble, France).
After obtaining the expected size of DNA chain (examined on agarose gel), isolated band should be used for DNA sequencing.
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