Here she was, just hours after selecting an outfit for her first day at a new high school: bleeding, barely conscious, cut down in an American crossfire.
On Friday, a half-hour after selecting Matteau, he said the subject was coming up again, but in a good way.
Infected cells were selected after 48 hours with puromycin (0.5 µg/ml).
To label glutamatergic neurons, E13.5 NEX-Cre embryos were electroporated with a pCAG-loxP-RFP-loxP-EGFP construct; single GFP-positive cells were then selected after 24 hours (Fig. 1A,B).
Just listen to the program hosts and their selected guests hour after hour: the Fed's QE 3 is futile, won't work, can't work, will surely backfire, will undoubtedly cause rampant inflation, is wrongheaded, delusional, incompetent, or worse.
Positive transfectants were selected 24 hours after transfection using puromycin for 24 more hours.
Flies of the indicated genotypes were selected 144 hours after eclosion and their fat-bodies and mid-guts were dissected.
Cell culture medium was supplemented with 2 µg/ml puromycin after 48 hours to select against non-transfected cells.
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After 48 hours, we selected red-fluorescent cells using FACS and carried out Western blot on the protein extracted from the fluorescent cells.
To assay larval growth, cohorts of 20 larvae of each genotype that had hatched were selected randomly after 24 hours.
