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After seeding with labelled cells (PKH26+), these denuded areas of the cryopreserved artery were entirely covered by fluorescent cells.
After seeding with hematopoietic stem cells (HSCs), ICC scaffolds were capable of supporting expansion of CD34+ HSCs with B-lymphocyte differentiation.
Resorbable porous ceramic constructs, based on silicon-stabilized tricalcium phosphate, were implanted in critical-size defects of sheep tibias, either alone or after seeding with bone marrow stromal cells (BMSC).
After seeding with chondrocytes and culture in vitro for 8 weeks, the cell-LDTM constructs formed tubular cartilage with relatively homogenous cell distribution in both micropores and bilateral surfaces.
Rifampicin-resistant attenuated E. coli O157 H7 ATCC 700728 (BLS1) was inoculated onto the soil beds after seeding with a backpack sprayer or onto 2- or 4-week-old lettuce plant foliage with a spray bottle at a level of 7 log CFU ml−1.
Cells spread on the protein lines were all fibroblast-like within 24 hours after seeding with their long axes preferentially parallel to the pattern lines.
Similar(36)
The TEER value in this culture appears to be strongly related to the amount of FCS in the medium from day 3 4 after seeding onwards, with cultures that were incubated with less FCS having higher TEERs.
The addition of 10% CS in the biomaterial composition increased the LDH levels with approximately 34% at 4 days after seeding and with approx. 45% after 7 days of culture.
Recombinant Wnt5a of 200 ng/ml (R&D Systems, Minneapolis, MN, USA) was added immediately after seeding together with the FZD7 siRNA transfection reagents as described above.
Caco-2 cells were seeded upon 10 mm coverslips coated with 20 μg/ml fibronectin in a 48-well plate at either low density or high density, and 2 h after seeding treated with the siRNA/transfection mix.
To highlight the importance of protecting electrodes during the seeding of "sticky" anchorage-dependent stellate cells, aptamer-functionalized electrodes were characterized by SWV after seeding cells with or without microcup protection.
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