Sentence examples for after quantitation from inspiring English sources

This was calculated after quantitation of the total DNA content and of the cell density of a culture aliquot.

30 µg of liver protein was loaded onto a 10% polyacrylamide gel after quantitation using a QuantiPro BCA assay (Sigma Aldrich, cat: QPBCA-1KT).

After quantitation 10 µg of digested DNA was separated on a 0.8% agarose gel overnight at 0.10 V/cm, subjected to partial acid hydrolysis and transferred to positively charged nylon membranes (Roche, Indianapolis IN).

After quantitation of each target and reference genes by the standard curve method, relative expression was calculated to compare the value of cell lines with the average value of HBEC 15 and 21 cell lines (for NSCLCs lines) or the value of human adult normal colon RNA (BioChain Institute, CA, USA) for CRC lines, respectively.

After quantitation of protein content by the Dc protein assay kit (BioRad Laboratories, Hercules, CA), 60 µg of protein for each sample was separated on a NuPAGE 12% Bis/Tris MOPS buffer protein gel (Invitrogen) and blotted onto nitrocellulose membrane (0.45-µm pore) as directed by the manufacturers.

After quantitation of this material along with [2-3H]DLO, [2-3H]glycoproteins and [2-3H]fOS, it was noted that when total cellular [2-3H]mannose isconsideredn is considered, [2-3H]mannose labelled material in the FA eluates corresponded to ∼4% of total cellular radioactivity in EBV ctrl 1 cells, and this value increased 3 fold in EBV CDG Ig cells (Fig. 2B, left hand panel).

All peptides with an IonsScore ≥25 were quantified using MS Quant (v1.5) [49]; after automated quantitation all files were manually edited to ensure consistent quantitation and the peak area ratios were exported for further analysis.

After Ribogreen quantitation, 500 ng of RNA was used in a random primed first strand synthesis reaction: Five hundred ng RNA was mixed with 1 µL 250 ng/µL random primers (Invitrogen 48190-011), 1 µL dNTP's (Invitrogen 18427-013), and water for 10 µL total volume.

After KAPA quantitation and dilution, the library was sequenced on an Illumina HiSeq 2000 with 101 bp paired-end reads.

After KAPA quantitation and dilution, the library was sequenced on an Illumina HiSeq 2000 with 100-bp paired-end reads.

After KAPA quantitation and dilution, the library was sequenced on Illumina HiSeq 2000 to generate 101 bp paired-end reads.