Sentence examples for after precipitated from inspiring English sources

PH-TP was dissolved in warm water, were subjected to a sequential precipitation with ethanol at 40%, 60%and90%0% (the supernatant after precipitated by 60percentt of ethanol) to obtain three fractions of FP40, FP60 and FP90 (the small molecules polysaccharide).

The peptide was cleaved from the resin and deprotected by treatment with a mixture of trifluoroacetic acid (TFA) and scavengers and after precipitated in ice-cold ethylic ether.

After precipitated by the protein A/G beads, the enriched mitochondrial proteins were subjected to Western blot with KIAA0101 rabbit antibody.

After precipitated by isopropanol, cassettes were ligated by adding 1 × ligation buffer, 10 units ligase (TaKaRa) and 500 ng Sau3AI cassettes or 50 ng EcoRI, HindIII, XbaI, SalI, or PstI cassettes in 30 μL reaction mix with incubation for 45 min at 16°C.

Finally, the enriched adzuki bean flavonoids (ABF) were obtained from the supernatant after precipitating with methanol acetone.

To detect putative translation products regardless of their size or heterogeneity, we analyzed the radiolabeled material after precipitating the polypeptides with 25% trichloroacetic acid.

Sex steroids were measured from cryobanked saliva after precipitating out all cell and particulate components.

After precipitating the supernatant, the DNA was completely digested by restriction enzymes.

High density lipoprotein was measured after precipitating VLDL and LDL cholesterol in the presence of magnesium ions.

After precipitating for 30 min at 37°C, the mixtures were centrifuged for 15 min 12,000 g and 4°C.

39 Interestingly, we observed dark and intense PCR bands after precipitating Dnmt3a and Dnmt3b in both E7- and PC control-transfected cells.