Exact(8)
Raman spectra indicate that peaks for untreated PET fabric are disappeared after plating with copper.
As shown in Figure 4B and 4C, the outgrowth from plated tissue is established 5 days after plating with no detectable GFP fluorescence.
The medium was replaced 2 hours after plating with neurobasal medium (Gibco) supplemented with B27 (Invitrogen), 2 mM Glutamax and Penicillin/Streptomycin. Hippocampal neurons were transfected using Lipofectamine 2000 (Invitrogen) at 10 14 DIV and fixed 2 days after transfection.
Cells were transfected 18 24 h after plating with 240 ng of the SuperTOPFlash reporter [58] and 10 ng pRL-TK (Clontech) using Fugene 6 (Roche) transfection reagent in a 3∶1 (v/w) ratio of reagent to DNA.
Interestingly, this was also true when comparing MEFs irradiated with increasing single doses of X-ray 24 h after plating with MEFs irradiated 48 h after plating (Fig. 1B).
Cells were transfected one day after plating with the following plasmids: NE and EC (150 or 400 ng each/well), a reporter plasmid expressing EGFP after Cre-mediated DNA recombination (400 ng/well) and in some cases (Fig. 5A, B) with pCMV-DsRedNuc (100 ng/well) using Lipofectamin 2000 (Invitrogen).
The cell image was traced immediately after plating with a camera-equipped microscope (Carl Zeiss Microscopy).
MNs were transduced at 24 h after plating with multiplicity of infection (MOI) of 10.
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