after permeabilization with

USAGE SUMMARY

The phrase "after permeabilization with" is correct and usable in written English.
It is typically used in scientific or technical contexts to describe a process that follows the treatment of a sample to make its membrane permeable. Example: "After permeabilization with Triton X-100, the cells were stained with a fluorescent dye."

✓ Grammatically correct

Science

Human-verified examples from authoritative sources

Exact Expressions

60 human-written examples

Labeled IPMCs were rarely seen on saponin-permeabilized cells, but after permeabilization with Triton X-100, most IPMCs were strongly stained by the PI 4,5 P2-specific antibody (Additional file 5).

BMC Biology

The organizations of both MTs and actin cytoskeletons were retained intact even after permeabilization with this buffer.

Biochemical and Biophysical Research Communications

After permeabilization with IC staining buffer, thymocytes were further incubated for 1 h at 37°C with 50 µl of the TUNEL solution or control solution.

Plosone

After permeabilization with 0.5% Triton-PBS, total bacteria were labeled with rabbit anti-Ochrobactrum antibodies followed by TRITC conjugated anti-rabbit antibodies.

Plosone

After permeabilization with 0.5% saponin solution for 10 min at 4°C cells were stained with PE-conjugated monoclonal antibodies directed against the respective cytokines.

Plosone

After permeabilization with 0.2% saponin (Sigma), cells were incubated with anti-phospho-PKC delta (Santa Cruz), followed by staining with Alexa Flour 488 anti-goat IgG (Molecular Probes).

Plosone

After permeabilization with PBS containing 0.1% Triton X-100 for 10 minutes sections were blocked for 1 hour with PBT (0.15% BSA, 0.1% Tween 20 in PBS).

Plosone

After permeabilization with 0.2% Triton®-X 100 for 15 minutes, lysosomal compartments were stained with anti-LAMP-1 mouse monoclonal antibody (Santa Cruz Biotechnology, clone H5G11) [35] followed by Alexa-488-labeled goat anti-mouse secondary antibody (Molecular Probes).

Plosone

After permeabilization with PBS containing 0.1% Triton-X, cells were washed twice with PBS-T, blocked with PBS containing 3% BSA for 1 hour at room temperature, and incubated with primary antibody (Santa Cruz, 1∶200) overnight at 4°C.

Plosone

For BrdU-treated embryos, DNA was denatured after permeabilization with 0.25 N HCl and 0.5% Triton X-100 for 20 min at room temperature and washed extensively in PBS with 1.5% BSA.

Plosone

One or two hours after UVB irradiation, keratinocytes were fixed with 100% methanol at −20°C for 15 min. After permeabilization with 0.5% Triton X-100 and blocking, cells were incubated with a monoclonal anti-TC-PTP antibody (R&D Systems Inc).

Plosone

Expert writing Tips

Best practice

When describing a scientific protocol, clearly specify the permeabilizing agent and its concentration following the phrase "after permeabilization with" to ensure reproducibility.

Common error

Avoid vague statements. Instead of just saying "after permeabilization with solution", specify the exact solution, concentration, and duration for clarity and replicability.

Linguistic Context

The phrase "after permeabilization with" functions as a prepositional phrase indicating the temporal sequence in a scientific or technical procedure. As Ludwig AI explains, it sets the stage for actions performed once a membrane has been rendered permeable.

Expression frequency: Very common

Frequent in

Science

100%

Less common in

News & Media

0%

Formal & Business

0%

Ludwig's WRAP-UP

In summary, "after permeabilization with" is a prepositional phrase correctly and commonly used in scientific and technical writing to denote the sequence of steps in a protocol. As Ludwig AI confirms, the phrase is grammatically sound. Its primary function is to indicate that a specific action is performed following the permeabilization of a sample. While alternatives like "following permeabilization with" exist, the original phrase remains a prevalent choice within scientific literature. Ensure you specify the permeabilizing agent and its concentration for clear communication.

More alternative expressions

Phrases that express similar concepts, ordered by semantic similarity:

following permeabilization with

Uses 'following' instead of 'after', maintaining a similar temporal relationship.

subsequent to permeabilization with

Replaces 'after' with 'subsequent to', providing a more formal tone.

post-permeabilization with

Uses 'post-' as a prefix to indicate the time frame after permeabilization.

upon permeabilization with

Changes the preposition to 'upon', indicating something happening immediately after.

once permeabilized with

Emphasizes the condition of being permeabilized before the subsequent action.

permeabilized with, then

Explicitly states the permeabilization and then introduces the subsequent action.

with permeabilization complete, using

Highlights the completion of permeabilization before the following steps.

after treatment for permeabilization with

Adds 'treatment' and rephrases to clarify the process.

following the permeabilization procedure with

Specifies a permeabilization procedure, adding detail.

subsequent steps with permeabilization using

Reorders the phrase to emphasize subsequent steps after using a permeabilizing agent.

FAQs

How can I use "after permeabilization with" in a sentence?

You can use "after permeabilization with" to describe a step in a scientific procedure, for example, "After permeabilization with Triton X-100, the cells were incubated with antibodies."

What is a good alternative to "after permeabilization with"?

Alternatives include "following permeabilization with", "subsequent to permeabilization with", or "post-permeabilization with".

Is it better to say "after permeabilization with" or "following permeabilization with"?

Both "after permeabilization with" and "following permeabilization with" are grammatically correct and largely interchangeable, though "following" may sound slightly more formal.

What does permeabilization achieve in the context of using "after permeabilization with"?

Permeabilization makes cell membranes permeable, allowing reagents like antibodies to access intracellular components. The phrase "after permeabilization with" indicates the next step taken once this access is achieved.