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Micelle formation is supposed to be disturbed by interaction with aggregating protein molecules; after neutralisation of BSA surface charge, gemini surfactant molecules could further bind to BSA.
The pericardial blood was suctioned in a separate reservoir, treated through an autologous transfusion system and re-transfused into the CPB circuit or directly to the patient after neutralisation of heparin for On-pump CABG.
After neutralisation of endogenous peroxidase in a phosphate-buffered saline (PBS) solution containing 0.1% hydrogen peroxide (H2O2) for 5 min, slides were incubated overnight at +4°C with anti-TPO monoclonal antibody (MoAb 47 clone; Dako, Glostrup, Denmark) diluted to 1 : 100 (2 μg ml−1).
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After neutralisation with 0.1 volume of 1 M Tris, pH 9.5, GM-Fc was dialysed against PBS and protein concentration determined by the bicinchoninic acid method (Pierce).
In method 3, we further reduced the kit volume by subdividing the kit into 260-μL freeze-dried aliquots, each able to reduce up to 1 GBq [99mTcO4]- in 100 μL resulting in a radioactivity concentration of approximately 8 GBq/mL after neutralisation.
To measure the hepatic glycogen content, liver tissue was digested in an alkaline medium and, after neutralisation, incubated in the absence or presence of amyloglucosidase.
DNA which remained double-stranded after 30 min of alkaline treatment was detected after neutralisation and immediate fragmentation followed by binding to the Hoechst 33258 dye (bisbenzimide) and fluorescence measures.
The accelerated negative ions pass through a neutraliser, then an electrostatic deflection system, where residual ions, exiting after neutralisation, are dumped.
After neutralisation and lyophilisation, these intracellular metabolite extract samples were re-suspended in 1 ml of D2O for H NMR analysis.
After neutralisation with potassium dihydrogen phosphate (KH2PO4) and centrifugation, nucleosides were determined by HPLC.
After neutralisation with 15 μl 1 M Tris-HCl, pH 9.1, the eluted phage were amplified by infecting 20 ml of a 1 100 dilution of an overnight culture of E. coli ER2738.
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