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Viability and proliferative capacity of the cells were significantly decreased (2 3 orders of magnitude) after internalization of Dox loaded BGs as compared to cells incubated with free Dox.
It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG.
The morphology of HUVEC cells did not vary after internalization of MSNs, indicating that the MSNs are not only a biocompatible delivery system for growth factors/proteins, but also an ideal biomaterial for the application in bio-imaging.
Immediately after internalization of the particle, the viral RNA is released.
Indeed, we found that miRNAs highly expressed within MVs were transferred in mTEC after internalization of MVs.
This decreased tumor uptake at longer time points is potentially indicative of a slow rate of metabolism and excretion of 89Zr4+ after internalization of HER2/neu-bound 89Zr-DFO-trastuzumab (Figures S4 and S5, and Table S4).
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After internalization, reduction of the cross-linked peptide DNA condensates yielded increased transfection efficiencies into different cell types cultured in 2D sandwich assays, and comparable values for HUVECs cultured in a 3D fibrin matrix, as compared to PLL DNA condensates.
Immediately after the internalization of the virus particle, genome release takes place from vesicles or tightly sealed membrane invaginations located within 100 200 nm of the plasma membrane.
Horwitz et al. reported an association between L. pneumophila containing phagosomes and host cell mitochondria soon after the internalization of the bacteria, which were extended by the demonstration of physical contacts between the two compartments [9], [58].
Another possibility is that rupture occurs only after endocytic internalization of the intact EV particle.
No signs of cellular death were observed after prolonged internalization of Staphylococcus aureus 6850 in epithelial cells.
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