In addition, Springer and Horton describe in their methods the possibility of 'non-agglutinating-in-saline' ABO antibodies and their detection with anti-human serum after immunizing chickens with ABO antigens.
Similar results were obtained by Cubas et al. (2009) [ 40] after immunizing mice with SHIV VLPs SIV Gagg + HIV SF162 Env), since they observed an antibody response both to external envelope protein (Env) and to the internal gag protein.
To determine whether FTY720 was also ineffective in suppressing or preventing arthritis in immunocompetent BALB/c mice, we administered placebo or FTY720 orally to BALB/c mice after immunizing them with PG in DDA adjuvant to induce the primary form of PGIA.
In the present study, we show that antibodies elicited after immunizing a rabbit with the peptide 131 151 of the U1-70K protein cross-react with the short RNP1 motif contained within the immunizing sequence but significantly less well with the RNP1 motifs of U1-A and hnRNP-A2 proteins, indicating that in solution these short peptides do adopt different conformations.
After immunizing New Zealand rabbits with the immunogen, the ADCC reporter gene was activated, suggesting the existence of ADCC activity.
After immunizing the chickens with rVP60-N, the anti-rVP60-N IgY was isolated, and the activity and specificity of the IgY antibody were analyzed by ELISA and Western blotting.
T cell lines specific for immunogenic determinants of AQP4 were generated from draining lymph node cells isolated 2 weeks after immunization from mice immunized with AQP422 36/CFA or AQP4289 303/CFA.
Fifteen days after the third immunization, mice immunized with mTS-IMX showed a TS-specific IgG response with titers >106 and high avidity, an increased IgG2a/IgG1 ratio, significant delayed-type hypersensitivity (DTH) reactivity, a balanced production of IFN-γ and IL-10 by splenocytes and a strong IFN-γ secretion by CD8+ T lymphocytes.
The antibody response in both strains reached a saturation plateau after the second immunization when immunized with the highest vaccine dose tested.
Sera from the three groups collected after two immunizations, including mice immunized with DCB6AdTA, stained EL4 murine cells (Fig. 5A).
