Sentence examples for after gene expression from inspiring English sources

After gene expression in E. coli, the recombinant proteins were purified from the cell-free extracts in a two-step procedure that makes use of: (i) Affinity chromatography on a HisTrap FF column (GE Healthcare, Chicago, Illinois, United States, 5 mL column) and (ii) Anion exchange chromatography on a ResQ column (GE Healthcare, Chicago, Illinois, United States 6 ml column volume).

After gene expression data from all groups (hESC, hiPSCs and donor cells) were subjected to the same statistical screening criterion (P-value cut off <0.05 and a fold-change cut off of 2.0), we then calculated the distances among them.

After gene expression analysis of the samples, marker genes can be selected according to the correlation of their expression to cell concentration.

After gene expression quantification, differential gene expression analysis was conducted using DESeq2 version 3 in R. Following the DESeq2 guidelines, absolute read counts per gene as obtained from Sailfish were normalized between samples to account for differences in sequencing depth.

In general, the Tet-On system is preferred over Tet-Off for gene therapeutic applications because its responsiveness is faster and because there is no need for continuous pharmacological treatment after gene expression termination.

Previous studies have documented an association between VEGF expression and malignancy after gene expression profiling of a very small case series comprising only 20 cases, with 9 malignant tumours (Favier et al, 2002), and another used immunohistochemistry of paraffin-embedded samples in a single-centre, retrospective study of 105 tumours, only 8 of which were malignant (Salmenkivi et al, 2003).

After gene-expression profiling, we identified and validated gene-expression signatures that define two distinct subtypes of tumor vasculature, an understanding of which would represent an important step toward the improved design and targeting of therapeutic modalities.

After calculating gene expression levels, the differentially expressed genes (DEGs) were screened by comparing gene expression levels.

Gene expression clusters were identified after weighted gene expression network creation using the WGCNA package in R [ 31].

Arnold, L. W., Pennell, C. A., McCray, S. K. & Clarke, S. H. Development of B-1 cells: segregation of phosphatidyl choline-specific B cells to the B-1 population occurs after immunoglobulin gene expression.

In order to circumvent the difficulty encountered in the expression and purification of the recombinant products in E. coli system, we have developed a novel and facile method of removing the polyhistidine tag from target proteins after heterologous gene expression.