The phrase "after fork" is not correct and lacks clarity in written English.
It may be intended to refer to a point in time or a process that occurs following a decision or divergence, but it needs more context to be usable.
Example: "We will proceed with the project after fork, ensuring all team members are aligned."
Alternatives: "following the fork" or "post-fork".
Therefore, our results suggest that Swi3 has a specific role in replication recovery after fork breakage, which is independent of Cds1 or Chk1 activation.
To investigate the effect of Swi3 mutations on replication fork stability, we examined the recovery of DNA replication after fork breakage induced by CPT treatment.
Since Swi3 is important for the full activation of Cds1 and for the stabilization of stalled replication forks in response to HU that activates Cds1 [20], we also monitored replication recovery after fork arrest due to HU exposure.
However, intact chromosomes from all swi3 mutant cells failed to migrate into the gel at 1.5 h and 3 h during recovery, indicating that Swi3 is required for the recovery of DNA replication after fork breakage.
Taken together with the fact that swi3-E39 (Class III) mutants failed to recover replication after fork collapse provoked by CPT (Figure 5A, middle panel), our results also indicate that Swi3's role in Cds1 activation is independent of the function of Swi3 in the recovery of broken replication forks.
Moreover, considering the fact that swi3-E39 has defects in replication recovery after fork breakage but not in Cds1 activation (Figure 5A and 6A), our results are consistent with the notion that the checkpoint role of Swi3 is not sufficient for proper establishment of sister chromatid cohesion.
After forking out for a wedding, you might not want to shell out a fortune on a holiday.
There have been times when I have felt cheated after forking out for a book only to find a particular signature dish wasn't included.
My overwintering foliage also needs a good start in life, and after forking the site over I sprinkled seaweed meal over the ground.
After forking down my money (there was a line, but at this point, I didn't even notice), I entered to try to find the "Vietnam" exhibition.
After forking over $65,000 on the house they just sold, they are struggling with $670,000 in debt on their present, larger home — perhaps more than the house itself is worth.
