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MAP2+ neurons and GFAP+ glia could be detected after differentiated for more than 35 days.
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Total RNA of OPCs grown on PLL control or MPE substrates with various concentrations (0.4 µg MPE, 4 µg MPE and 40 µg MPE) was harvested after the cells were differentiated for 3 days using RNeasy Mini Kit (Quiagen, Hilden, Germany) according to the manufacturer's instruction.
Six days after irradiation, primary cultures were differentiated for 7 days to evaluate the effect of γ rays on the multipotentiality of the neural precursors.
After transfection, the cells were differentiated for 48 h in DMEM containing 1.0 g/l glucose supplemented with 2% (v/v) FBS and 2 mM dbcAMP with or without CysC, 3-methyladenine (3-MA; Sigma), CC (EMD Millipore), AICA-riboside (AICAR; EMD Millipore) or CA-074 methyl ester (EMD Millipore).
Fig. 3 Daily residual load before and after flexible power generation from biomass, differentiated for winter and summer time.
To examine the functional properties of dopaminergic neurons derived from ESCs/NSCs cultured in defined medium, we transplanted cells that had been differentiated for 20 days (after the NSC stage) into the striatum of rats.
Non-adherent cells were collected after 24 hours, seeded on coverslips and differentiated for 7 days in polystyrene culture plates.
In an initial experiment, H1 HESCs were differentiated for 30 days with RNA samples taken before and after differentiation.
After 8 10 DIV the neurospheres or spheroids were differentiated for 5 days in the absence of growth factors on PDL and laminin-coated chamber slides.
Cells expressing the wild-type or mutant myogenin promoters were differentiated for 2 days before being transfected with AdT or AdC (control adenoviral vector) after which cells were differentiated for a further 2 days.
hNPCs were differentiated for 24 h.
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