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After clean up with the QIAquick PCR Purification Kit (Qiagen), the PCR products were used to perform a sequencing reaction, containing either the forward or the reverse M13 primer and Big Dye Terminator mix (Applied Biosystems).
Double stranded cDNA was obtained by using DNA ligase, DNA polymerase I and RNAse H at 16°C for 2 h, followed by T4 DNA polymerase at 16°C for 5 min. After clean up with a phase lock gel (Qiagen), ds-cDNA was used for in vitro transcription (IVT).
After clean up with a RNeasy kit (Qiagen), the biotin-labelled IVT-RNA was fragmented in a buffer containing 40 mM Tris-acetate (pH 8.1), 100 mM potassium-acetate and 30 mM magnesium-acetate, at 94°C for 35 min. RG-U34A arrays (Affymetrix, Santa Clara CA, USA) were used, representing ~7000 transcripts, of which 6399 had a unique LocusLink identification number (ID) at the time of data analysis.
Thick oil is revealed on the beach at Grand Isle's State Park after clean up crews use an auger to take 30 inch deep core samples that show oil from the deepwater horizon oil spill exists on several levels beneath the sand.
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Donors can deduct the sites' full market value as charitable contributions, and can advise the foundation on the charities that would receive the proceeds from the sale -- after clean-up costs and foundation handling fees are deducted.
Extracts were analyzed by gas chromatography (GC-MS and GC-FID) after clean-up and concentration.
Recoveries obtained after clean-up using OASIS HLB® and IAC were in the range of 74 104% and 60 85%, respectively.
After clean-up (see "GPC") of NaOH-extractable radioactivity from the 5 cm top soil layer, extracts from all soil samples were analyzed by GPC.
––– Joel GolBut Sthen weiter, VICE UK.
After clean-up this occurred for three pesticides only.
After clean-up, 100 ng of each probe was labeled with [γ-P]dCTP.
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