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Heterotrimers were purified from CHO-conditioned media after buffer exchange to phosphate-buffered saline (PBS) (pH 7.4) by ultrafiltration/diafiltration and loading onto an immobilized metal affinity chromatography NI-NTA His Bind Superflow Column (Novagen, Gibbstown, NJ, USA).
After Buffer, instead of working on "the next hot app," Sun wanted to go in a different direction.
After buffer exchange, the His-tag was cleaved by a tobacco etch virus (TEV) treatment.
The protein solution after buffer exchange was subjected to the peptide-cleavage process.
Analysis of seepage rates into an open borehole indicate the presence of an EDZ prior to the emplacement and some 'healing' of the EDZ after buffer placement.
Figure 1 stage IV and Fig. 2c shows that the layer was stable and that could not be easily rinsed away after buffer rinsing.
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The control performance is further improved after a buffer tank is installed upstream of the process.
The fluid-diffusion in the 3rd and 4th chambers mainly happens after the buffer period.
After the Buffer Growing process, a list of potential candidates for the matching reference is computed.
Comparison of the aspect ratio before and after the buffer layer growth.
After the buffer was changed to separation buffer, the mixture was incubated at 37°C for 10 min.
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CEO of Professional Science Editing for Scientists @ prosciediting.com