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The yellow areas, typifying the co-localization, were found to locate either in early endosomes or in late endosomes after incubation with BLPs, clearly stating that BLPs after being internalized into cells is experiencing membrane-associated protein-coated pit invagination to form endosomes.
If CD does enter the brain, it presumably could exert its impact on cholesterol and GSL accumulation by acting at the plasmalemma of brain cells or after being internalized.
Taken together, these data suggest that after being internalized and retrotranslocated into the cytosol, Firefly and Renilla luciferases could potentially undergo refolding in the DC-like cell line KG-1.
In Dab2/clathrin-containing endosomes, Wnt3A-mediated phosphorylation of LRP6 at S1490 does not occur, suggesting that after being internalized these Dab2 vesicles fail to engage the β-catenin destruction complex and its associated CKI and GSK3β kinases to phosphorylate LRP6.
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The amount of internalized EGFR increased gradually over 5 20 min period with about 70% of EGFR being internalized after 20 min of stimulation.
Those without cytotoxicity, like B cells or monocytes underwent typical entosis after they were internalized into tumor cells.
While free Dox is taken up via passive diffusion and is active immediately, we designed our nanoparticles to release active Dox only after they are internalized and are exposed to the acidic environment of the endocytic vesicles.
BoNT LC translocation requires low endosomal pH; after toxins are internalized into SVs/endosomes via endocytosis, blockade of acidification at different time points by con A traps the remaining LCs within the SVs/endosomes, thereby preventing SNARE cleavage by LCs in the cytosol.
cAMP levels returned to baseline after the particle was internalized.
This demonstrates that insulin receptors recovered in the endosome fraction after insulin stimulation were internalized, and therefore cannot come from contaminating plasma membrane fragments or caveolae that have been torn off the plasma membrane during the homogenization and subcellular fractionation of the adipocytes; and that we therefore can refer to this fraction as an endosome fraction.
This feature enables this RIP to bind to glycoproteins and glycolipids on the cell surface, after which it is internalized by different mechanisms [ 3].
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