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Figure S1 C shows a picture of the standing cultures after 12 hour incubation, where precipitation of the bacteria grown at starting pH of 6.4 can be observed.
After 12 hour overnight fast, the experiment was started at 8: 00 am with basal blood sampling and basal MEG measurements.
After 12 hour incubation, each set was treated with sulindac sulfide for 24 hours.
In this model non-human primates Baboon (Papio hamadryas sp). is induced with high sugar, high fat diet after 12 hour fasting.
Incubation of MCF-7 with IC50 concentration of EEC after 12 hour reduced the cell viability to 26.45 ± 0.41% with 31.92 ± 0.48% and 23.98 ± 0.45% of early apoptosis and late apoptosis, respectively.
Blood samples were obtained after 12 hour overnight fasting, kept at 4°C for 1 h, and centrifuged at 3000 rpm for 10 m' at 4°C to obtain serum or plasma.
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No carbon deposition was detected after 72 hours.
a after 2 hours of NPPV.
Nasal secretion began after 12 hours.
Variables, including systemic haemodynamics were recorded at start study (T1), after 12 hours (T2) and after 24 hours (T3).
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