Exact(2)
In addition to the aforementioned strain, the same group also constructed a strain with the same tod-lux complex inserted in the chromosome (TVA8).
The GPDpr-HSV-TK/ ADH1 pr-hENT LEU2 ("BrdU-Inc") cassette was integrated into the aforementioned strain by transforming with PacI-digested p405-BrdU-Inc plasmid (Viggiani and Aparicio 2006) and selecting for leucine prototrophy.
Similar(58)
Finally, measurements of mitochondrial content of the aforementioned strains experimentally address the question whether alternative respiration leads to changes in mitochondrial biogenesis.
Figures 4A and 4B plot the results obtained using human PBMC monocytes from one G6PD-deficient patient who was infected with both of the aforementioned strains of DENV-2.
However, these data offer no explanation for the physiological differences between the aforementioned strains.
All but three investigated human commensal and all animal strains possessed MLST types different from hospital-associated strains; all but the three aforementioned strains were IS 16 negative.
Thus, it can be expected that compounds that cause oxidative stress will be detected to some extent by the aforementioned strains.
In addition to the aforementioned strains, EBHmp (hmp) and PGRFM (pgi) have been developed; they contain promoters that belong neither to the OxyR pathway nor to the SoxRS pathway.
All the aforementioned strains were plated on 4 % w/v tryptic soy agar (Sigma-Aldrich, Saint-Louis, MO, USA), apart from (1) Pfl29and AviF08190819, which were plated on 2.5 % w/v lysogeny broth (Sigma-Aldrich) containing 1.5 % w/v agar for microbiology (Sigma-Aldrich) and (2) andSP245 and BceA0145, which were grown on modified lysogeny broth agar supplemented with 2.5 mM CaCl2 and 2.5 mM MgSO4.
To validate various assumptions of the assay, we subjected the aforementioned qCTF strain grown in media nonselective for the MC to flow cytometry analysis.
To determine whether a DSB mediated by I- SceI can increase transformation and recombination efficiency in T. reesei, the aforementioned reporter strain JP7.7.12 was transformed with a linear fragment comprising a glucoamylase expression cassette (Fig. 4).
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